Essential Active-Site Lysine of Brain Glutamate Dehydrogenase Isoproteins
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چکیده
منابع مشابه
Photoaffinity labeling of brain glutamate dehydrogenase isoproteins with an azido-ADP.
The ADP binding site within two types of bovine brain glutamate dehydrogenase isoproteins (GDH I and GDH II) was identified using photoaffinity labeling with [alpha-32P]8-azidoadenosine 5'-diphosphate (8N3ADP). 8N3ADP, without photolysis, mimicked the activatory properties of ADP on GDH I and GDH II activities, although maximal activity with 8N3ADP was about 75% of maximal ADP-stimulated activi...
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Saccharopine dehydrogenase (e-N-(L-glutaryl-2)-L1ysine:NAD oxidoreductase (L-lysine-forming) EC 1.5.1.7) is inhibited by a variety of sulfhydryl reagents. Althoughp-chloromercuribenzoate and 5,5’-dithiobis(2nitrobenzoate) react with three sulfhydryl groups present in the enzyme, reaction with one reactive sulfhydryl group leads to complete loss of catalytic activity. Amino acid analysis of the ...
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Egg-white avidin was treated with 1 -fluoro-2,4-dinitrobenzene. Modification of an average of one lysine residue per avidin subunit caused the complete loss of biotin binding. Tryptic peptides obtained from the 2,4-dinitrophenylated avidin were fractionated by reversed-phase h.p.l.c. Three peptides contained the 2,4-dinitrophenyl group. Amino acid analysis revealed that lysine residues 45, 94 a...
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ژورنال
عنوان ژورنال: Journal of Neurochemistry
سال: 2002
ISSN: 0022-3042,1471-4159
DOI: 10.1046/j.1471-4159.1997.69010418.x